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KMID : 0903520060490040304
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2006 Volume.49 No. 4 p.304 ~ p.308
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Effect of Protease on the Extraction and Properties of the Protein from Silkworm pupa
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Kwon Hyo-Jung
Lee Kyoung-Hwan
Kim Jeung-Hoan
Chun Sung-Sook
Cho Young-Je
Cha Woen-Suep
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Abstract
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To extract insoluble proteins from silkworm pupa meal, the meal was treated with protease produced by Bacillus sp. JH-209. The extraction of insoluble silkworm pupa protein was enhanced at alkaline pHs ranged from 7 to 11 by treatment with the protease. The optimum extraction temperature was 40oC for in soluble protein treated with protease. The optimum protease treatment time for extraction of protein was 11 hrs and optimum amount of enzyme treated for extraction of protein was 60 Unit, respectively. The treatment of enzyme extracted more protein than ordinary extraction method without protease. The foaming capacity, foaming stability, emulsion capacity, and emulsion stability of silkworm pupa meal protein extracted by the treatment of the enzymes increased at all pH ranges. Further more oil absorption as well as water absorption capacities of the protein extracted by the treatment of the enzymes were also increased.
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KEYWORD
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extraction, physical properties, silkworm pupa protein, protease
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